The present study aimed to extract and purify the compound of Secoisolariciresinoldiglucoside oligomers (lignan) from flax seed (Linumusitatissimum) and its antioxidant activity. The Lignan was extracted by solvents which gave the best results were ethanol : 1,4 dioxane (1:1, v:v).SDG release after alkaline hydrolysisby using a methanolicNaOH , 20 mM, pH=8 at 50 ºC.followed by using following chromatographic techniques: Liquid-liquid, Sephadex LH-20 column chromatography, thin layer chromatographic (TLC), high performance liquid chromatographic (HPLC) and Fourier Transform Infra-Red(FTIR) . The EC50 values of Pure lignan extract (9 µg/ml) was shown possess DPPH radical scavenging activity compared to reference substances BHT and vitamin C (EC50= 3 and 4.2 µg/ml) respectively, and this was higher than partial pure lignan component (EC50= 25.5 µg/ml).The total phenolic content of the pure lignanwas higher than partial pure lignan which gave 22.312 and 14.85 ?g/ml respectively
CITATION STYLE
Al-Jumaily, E. F. (2013). Isolation and Purification of Secoisolariciresinoldiglucoside oligomers (Lignan) from Flax seed and its evaluation of antioxidant activity. IOSR Journal of Applied Chemistry, 5(6), 17–24. https://doi.org/10.9790/5736-0561724
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