The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells. © 2014 Springer Science+Business Media, LLC.
CITATION STYLE
Pyzocha, N. K., Ran, F. A., Hsu, P. D., & Zhang, F. (2014). RNA-guided genome editing of mammalian cells. Methods in Molecular Biology, 1114, 269–277. https://doi.org/10.1007/978-1-62703-761-7_17
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