Antibody Detection by Using a FRET-Based Protein Conformational Switch

Abstract

No wash, just go: Classical antibody-detection methods rely on heterogeneous detection schemes that involve multiple, time-consuming binding and washing steps. Here we present a new concept to translate the antigen-antibody interaction directly into a readily detectable fluorescent signal by using a single-chain sensor protein and taking advantage of the unique Y-shaped structure common to all antibodies. Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.