Cell line development aims to generate and select clones with desirable characteristics. One of the most important parameters for biopharmaceutical cell selection is cell-specific productivity (Qp) or the quantity of product produced per cell per day. Fluorescence-activated cell sorting (FACS) is a powerful, high-throughput technique that facilitates multiparametric characterization and isolation of individual cell clones from heterogeneous populations. Here, we describe a FACS-based method for section of high-producing CHO cell clones.
CITATION STYLE
Gallagher, C., & Kelly, P. S. (2017). Selection of high-producing clones using FACS for CHO cell line development. In Methods in Molecular Biology (Vol. 1603, pp. 143–152). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6972-2_9
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