Complete Genome of Multi-Drug Resistant Staphylococcus Aureus in Bovine Mastitic Milk in Anhui, China

Abstract

Staphylococcus aureus is a critical pathogen causing serious mastitis with huge economic losses in bovines. To reveal drug-resistant genes, a complete genome sequence of a multi-drug-resistant bacterium S. aureus derived from bovine mastitic milk was performed via High-throughput sequencing. Results revealed that the genome length of current S. aureus was 2.85 Mbp consisting of 2656 coding sequence genes, 60 tRNA, 19 rRNA, and 5 genomic islands. Functional annotation of S. aureus encoded proteins showed that 2090 (COG), 1586 (KEGG), 1600 (GO), 2648 (Refseq), 2300 (Pfam), 964 (SwissProt), and 1826 (TIGRFAMs) proteins were annotated. Antibiotic-resistant genes of tet, mepR, mepA, mgrA, norA, blaZ, arlS, arlR and LmrS in S. aureus genome were found via CARD annotation. More than 60 bacterial virulence factors including commonly known clfA, hla, hlb, fnbB, fnbA in S. aureus genome were uncovered through VFDB annotation. The current study may contribute towards effective treatment and developing new antibiotics against mastitis caused by S. aureus.