IC-RT-PCR Detection of Blackcurrant Reversion Associated Nepovirus, and Perspectives of Its Use for Diagnosis of the Reversion Disease

Abstract

A immunocapture-reverse transcriptase-PCR (IC-RT-PCR) method was developed to detected a new nepo-type virus isolated from a reverted blackcurrant (Ribes nigrum). IC-RT-PCR showed that this virus is assocd. both with the E and R forms of reversion disease originating from Scandinavia, Scotland, and New Zealand. Due to its origin and its consistent assocn. with the different forms of reversion, but not with any other viral disease of Ribes tested, the authors named the virus, blackcurrant reversion-assocd. virus (BRAV). [on SciFinder(R)]