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High-Resolution 3D Light Microscopy with STED and RESOLFT

  • Sahl S
  • Hell S
Springer International Publishing, (2019), 3-32
DOI: 10.1007/978-3-030-16638-0_1
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Abstract

We discuss the simple yet powerful ideas which have allowed to break the diffraction resolution limit of lens-based optical micros-copy. The basic principles and standard implementations of STED (stimulated emission depletion) and RESOLFT (reversible satura-ble/switchable optical linear (fluorescence) transitions) microscopy are introduced, followed by selected highlights of recent advances, including MINFLUX (minimal pho-ton fluxes) nanoscopy with molecule-size (~1 nm) resolution.

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Sahl, S. J., & Hell, S. W. (2019). High-Resolution 3D Light Microscopy with STED and RESOLFT. In High Resolution Imaging in Microscopy and Ophthalmology (pp. 3–32). Springer International Publishing. https://doi.org/10.1007/978-3-030-16638-0_1

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