Performance of petrifilm aerobic count plates on enumeration of lactic acid bacteria in fermented milks

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Abstract

This study aimed to compare Petrifilm Aerobic Count (AC) plates and the conventional pour plate methodology using the de Man-Rogosa-Sharpe (MRS) agar for the enumeration of lactic acid bacteria (LAB) in fermented milks (FMs), with different starter cultures added. FM samples (n = 66) were collected and plated on both methodologies, with incubation under anaerobic conditions at 35C for 48 h. The count results were compared by analysis of variance (P ≤ 0.05) and regression analysis. No differences between the mean counts obtained by both methodologies were observed, even when distinct FMs were compared. Considering all samples, a high correlation level was obtained between Petrifilm AC and MRS agar (r = 0.92), but these indexes were lower in FMs with Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus (r = 0.90) and Lactobacillus fortis (r = 0.81). Despite some slight interferences, Petrifilm AC has proven to be a convenient methodology on enumerating LAB in FM. © 2008, Blackwell Publishing.

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Nero, L. A., Rodrigues, L. D. A., Viçosa, G. N., & Ortolani, M. B. T. (2008). Performance of petrifilm aerobic count plates on enumeration of lactic acid bacteria in fermented milks. Journal of Rapid Methods and Automation in Microbiology, 16(2), 132–139. https://doi.org/10.1111/j.1745-4581.2008.00121.x

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