Integrin α3β1 - dependent β-catenin phosphorylation links epithelial smad signaling to cell contacts

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Abstract

Injury-initiated epithelial to mesenchymal transition (EMT) depends on contextual signals from the extracellular matrix, suggesting a role for integrin signaling. Primary epithelial cells defi cient in their prominent laminin receptor, α3β1, were found to have a markedly blunted EMT response to TGF-β1. A mechanism for this defect was explored in α3-null cells reconstituted with wild-type (wt) α3 or point mutants unable to engage laminin 5 (G163A) or epithelial cadherin (E-cadherin; H245A). After TGF-β1 stimulation, wt epithelial cells but not cells expressing the H245A mutant internalize complexes of E-cadherin and TGF-β1 receptors, generate phospho- Smad2 (p-Smad2) - pY654 -β-catenin complexes, and upregulate mesenchymal target genes. Although Smad2 phosphorylation is normal, p-Smad2 - pY654 -β-catenin complexes do not form in the absence of α3 or when α3β1 is mainly engaged on laminin 5 or E-cadherin in adherens junctions, leading to attenuated EMT. These fi ndings demonstrate that α3β1 coordinates cross talk between β-catenin and Smad signaling pathways as a function of extracellular contact cues and thereby regulates responses to TGF-β1 activation. © 2009 Kim et al.

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Kim, Y., Kugler, M. C., Wei, Y., Kim, K. K., Li, X., Brumwell, A. N., & Chapman, H. A. (2009). Integrin α3β1 - dependent β-catenin phosphorylation links epithelial smad signaling to cell contacts. Journal of Cell Biology, 184(2), 309–322. https://doi.org/10.1083/jcb.200806067

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