Regulation of gene expression by transcription factors is a fundamental mechanism in essentially all aspects of cellular processes. Transient expression assay of a reporter plasmid containing a reporter gene driven by a promoter of interest and an effector plasmid expressing a transcription factor has been a powerful tool for analyzing transcription factors. Here we present a protocol for polyethylene glycol (PEG)-mediated transformation of Arabidopsis protoplasts. It details preparation of protoplasts from Arabidopsis suspension cultured cells or leaves of soil-grown Arabidopsis plants and subsequent PEG-mediated transformation with reporter and effector plasmids. This protocol can be completed within 24 h from protoplast preparation to reporter assay. As an example, analysis of the membrane-bound transcription factor AtbZIP60 and its target BiP3 promoter is shown.
CITATION STYLE
Iwata, Y., Lee, M. H., & Koizumi, N. (2011). Analysis of a Transcription Factor Using Transient Assay in Arabidopsis Protoplasts. In Methods in Molecular Biology (Vol. 754, pp. 107–117). Humana Press Inc. https://doi.org/10.1007/978-1-61779-154-3_6
Mendeley helps you to discover research relevant for your work.