This chapter introduces a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual microdroplets manipulated on the microfluidic device were used as microvessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.
CITATION STYLE
Liu, J., Pyne, D. G., Abdelgawad, M., & Sun, Y. (2017). Appendix C: Automated vitrification of mammalian embryos on a digital microfluidic device. In Methods in Molecular Biology (Vol. 1568, pp. 309–316). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6828-2_23
Mendeley helps you to discover research relevant for your work.