Isolation and Identification of Endophytic Bacteria from the Andalas Plant Stem (Morus macroura Miq.)

Abstract

The number of death couse of infection continues to increase. Treatmentof infections is increasingly difficult due to increased resistance of germs to existingantimicrobial compounds. A new source of antimicrobial substances is needed. Onesource of antimicrobial substances is endophytic bacteria that live in the stems ofAndalas plants. The aim of this study was to compare the diversity of endophyticbacteria present in old and young Andalas plant stems. The method used to isolateendophytic bacteria from the stem of the Andalas plant is the streak plate method.Bacterial identification is done macroscopically and microscopically. The results ofmacroscopic observation showed that there are differences in the number of isolatesfound in old and young Andalas plant stems. 11 isolates of endophytic bacteria fromAndalas plant stems, each of which consists of 9 isolates of endophytic bacteria fromold Andalas stems and two isolates from young Andalas stems. A microscopicidentification was found that of the 11 isolates, eight isolates were gram-positive andthree isolates were gram-negative bacteria