The mouse olfactory epithelium is composed of a heterogeneous population of olfactory sensory neurons, where each neuron expresses one single type of odorant receptor gene, out of a repertoire of ~1000 different genes. Fluorescent-activated cell sorting (FACS) is a powerful technique, which can be used to isolate a cellular subpopulation from a heterogeneous tissue. The sorted neurons can then be used in gene expression studies, or analyzed for the presence of different DNA epigenetic modification marks. Here we describe a method to separate a subpopulation of olfactory sensory neurons expressing the odorant receptor Olfr17. In this method, the main olfactory epithelium from transgenic Olfr17-IRES-GFP mice is dissociated into single cells, followed by separation of the GFP positive cells by FACS.
CITATION STYLE
Leme Silva, A. G., Nagai, M. H., & Malnic, B. (2018). Fluorescence-Activated Cell Sorting of Olfactory Sensory Neuron Subpopulations. In Methods in Molecular Biology (Vol. 1820, pp. 69–76). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8609-5_6
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