Brevibacillus choshinensis is an excellent host for the production of secretory proteins. This host has also been applied successfully to effi cient production of CCN proteins. Described herein are methods of constructing plasmids for CCN protein production (IGFBP-, VWC-, TSP-, and CT-domain) with Brevibacillus as a host, cultivation methods for protein production, and methods of purifi cation for domain proteins using his-tag.
CITATION STYLE
Hanagata, H., & Mizukami, M. (2017). Production of recombinant CCN proteins by Brevibacillus choshinensis. In Methods in Molecular Biology (Vol. 1489, pp. 85–93). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6430-7_9
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