Isolation of compounds from the aqueous methanol extract of Cissus javana DC leaves and determination of its trace element content through wet digestion

Abstract

A new alcohol was isolated from the aqueous methanol extract of the leaves of Cissus javana DC which is widely used in Manipur, India for dissolution and expulsion of kidney stones. In addition, known compounds Stigmasterol, Stigmasterol glucoside, Onocer-7-ene 3a,21β-diol, β-amyrin- [olean12(13)-en-3-one] were also isolated from the same methanol extract. Analysis of trace element contents of the plant leaf reflects its role in the ethno medicinal property of the plant. It was found that the plant leaf have exceptionally high calcium (2960 mg/100 g), magnesium (465 mg/100 g) and iron (520 mg/100 g) content.