Minimal genome-wide human CRISPR-Cas9 library

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Abstract

CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other CRISPR-Cas9 libraries while preserving assay sensitivity and specificity. MinLibCas9 provides backward compatibility with existing datasets, increases the dynamic range of CRISPR-Cas9 screens and extends their application to complex models and assays.

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Gonçalves, E., Thomas, M., Behan, F. M., Picco, G., Pacini, C., Allen, F., … Garnett, M. J. (2021). Minimal genome-wide human CRISPR-Cas9 library. Genome Biology, 22(1). https://doi.org/10.1186/s13059-021-02268-4

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