Biacore™ systems (Biacore AB) enable label-free detection of molecular interactions in real time using surface plasmon resonance detection. Epitope mapping of antibodies is usually performed in a pairwise fashion where one antibody is used to capture the antigen from solution and the binding of a secondary antibody is monitored. In contrast to alternative approaches, the method allows for mapping of large matrices of antibody pairs without the need for cumbersome labeling steps, and the real-time analysis enables a better ranking of complex stability when compared with end-point assays. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
CITATION STYLE
Säfsten, P. (2009). Epitope mapping by surface plasmon resonance. Methods in Molecular Biology, 524, 67–76. https://doi.org/10.1007/978-1-59745-450-6_5
Mendeley helps you to discover research relevant for your work.