Isolation of Two Species of Chlamydomonas reinhardtii Flagellar Mutants, ida5 and ida6, that Lack a Newly Identified Heavy Chain of the Inner Dynein Arm

Abstract

Two novel Chlamydomonas mutants, ida5 and ida6, that lack subsets of innerarm dynein have been isolated and mapped to discrete loci on the right arm of linkage group XIV. Of the seven different innerarm dynein subspecies (a, b, c, d, e,/and g) identified by ion-exchange chromatography, ida5 lacks a, c, d and e, while ida6 lacks e alone; these are the only mutants that have been shown to lack subspecies e. Both strains can swim, albeit more slowly than the wild type. Hence, subspecies e must contribute to flagellar movement although it is unnecessary for the generation of undulating movement. © 1993, Japan Society for Cell Biology. All rights reserved.