Site-Directed Mutagenesis (SDM) allows for changes in the DNA sequence of plasmids using polymerase chain reaction (PCR). It is a reliable, accessible, and rapid method which is the common initial step of many biochemial or genetic experiments. Here we describe the various different forms of SDM before giving a detailed method for the introduction of substitutions, insertions, or deletions using a fast, ligation-free protocol, followed by colony PCR to screen for mutated sequences.
CITATION STYLE
McClellan, M. J. (2023). In Vitro Site Directed Mutagenesis. In Methods in Molecular Biology (Vol. 2633, pp. 87–95). Humana Press Inc. https://doi.org/10.1007/978-1-0716-3004-4_8
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