Frequency, amplitude, and propagation velocity of spontaneous Ca++-dependent contractile waves in intact adult rat cardiac muscle and isolated myocytes

Abstract

Spontaneous contractile waves due to spontaneous calcium cycling by the sarcoplasmic reticulum occur in unstimulated bulk rat papillary muscle and single rat cardiac myocytes with intact sarcolemmal function. We used video analytic techniques to quantify the wave characteristics in both bulk muscle and myocytes; laser-light scattering techniques were also employed in muscle. In muscle bathed in physiological concentrations of calcium, the true periodicity of these waves was a fraction of 1 Hz and increased up to several hertz with increases in cell calcium. This was paralleled by an increase in the frequency of scattered laser light intensity fluctuations. In myocytes, a range of spontaneous contractile wave frequencies similar to that which occurred in the muscle was observed; it could be demonstrated that an increase in superfusate calcium concentrations (2-15 mm at 23° ) increases the oscillation frequency but not amplitude. In both myocytes and muscle, low concentrations of caffeine (0.5 mm) and higher temperature increased the oscillation frequency but diminished their amplitude. However, the scattered light fluctuations did not change with temperature and decreased with caffeine. These results demonstrate that (1) the true frequency of spontaneous sarcoplasmic reticulum oscillations in the unstimulated rat muscle and single myocytes with intact sarcolemmal function is low, i.e., a fraction of a hertz; (2) with cell calcium loading, the oscillation frequency accelerates to those fequencies measured previously in the 'calcium overload' state; (3) while scattered light fluctuations which sample myofilament motion are a sensitive, noninvasive method of detecting the oscillations in bulk muscle, they can be insensitive to the divergent changes in oscillation amplitude and frequency.