The Argonaute (AGO) family of proteins plays an essential role in the process of microRNA (miRNA)-mediated gene silencing. More specifically, they are the only known proteins to associate directly with miRNAs within the RNA-induced silencing complex (RISC). Given the importance of miRNA regulation of the transcriptome and its vast implications for human disease, it is essential to understand the molecular underpinnings of miRNA-AGO interactions. Although there are methods available to investigate mature miRNA decay and loading onto AGO2, no feasible method exists to detail the opposite process: release of miRNA from associated AGO proteins. In this chapter, we describe in detail a methodology derived from biochemical approaches, which can be used to quantify the release of any given miRNA from AGOs.
CITATION STYLE
Min, K. W., Evans, J. G., Won, E. C., & Yoon, J. H. (2020). Detection of MicroRNAs released from argonautes. In Methods in Molecular Biology (Vol. 2106, pp. 151–159). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0231-7_9
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