Hepatoprotective activity of ethyl acetate fraction of Senggugu'S Root Bark (Clerodendrum Serratum L. Moon) on rats induced by carbon tetrachloride

Abstract

Our previous study showed that ethyl acetate fraction of Clerodendrum serratum L. Moon (EAFCS) root bark had in vitro antioxidant activity. The aim of this study was to evaluate hepatoprotective effect of EAFCR in rats induced by carbon tetrachloride (CCl 4 ). Thirty-six rats were randomly divided into 6 groups. Group 1 as control was given 0.5% Na-CMC. Group 2 as positive control were given silymarin at a dose of 100mg/Kg.BW. Group 3 as negative control were induced by CCl4. Group 4-6 as treatment groups were induced CCl 4 and given EAFCR at a dose of 25; 50 and 100mg/Kg BW, respectively. Biochemical and oxidative stress parameters in liver were determined. The results showed that serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and bilirubin were significantly lower, whereas the total protein was significantly higher after pretreatment with EAFCR at the dose of 100mg/Kg.BW (p < 0.05). Moreover, malondialdehyde (MDA) was significantly lower, whereas glutathione peroxidase (GPx) and catalase (CAT) were significantly higher after pretreatment with EAFCS at the dose of 100mg/Kg.BW (p < 0.05). In conclusion, EAFCR has potent hepatoprotective activity due to its antioxidant properties of the active compounds contained in this plant.