The quasispecies variation of hepatitis B virus (HBV) was believed to be a viral response to antiviral treatment and host immune pressure. Sanger sequencing was previously the classic approach for quasispecies analysis, but this method was also time-consuming and laborious. Ultra-deep sequencing has been widely used in viral quasispecies research, especially for low-frequency mutation detection. Here we present a multiple samples deep sequencing method employing the Illumina platform to detect HBV quasispecies variation in patient-derived samples.
CITATION STYLE
Long, Q. X., Hu, J. L., & Huang, A. L. (2017). Deep sequencing of the hepatitis b virus genome: Analysis of multiple samples by implementation of the illumina platform. In Methods in Molecular Biology (Vol. 1540, pp. 211–218). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6700-1_17
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