Expression of prokaryotic integral membrane proteins in E. coli

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Abstract

Production of prokaryotic membrane proteins for structural and functional studies in E. coli can be parallelized and miniaturized. All stages from cloning, expression, purification to detergent selection can be investigated using high-throughput techniques to rapidly and economically find tractable targets.

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Love, J. D. (2017). Expression of prokaryotic integral membrane proteins in E. coli. In Methods in Molecular Biology (Vol. 1586, pp. 265–278). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6887-9_17

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