Optimized Production of Bacteriocin from Cheaper Carbon and Nitrogen Sources Using Response Surface Methodology

Abstract

Background and Objective: Bacteriocins are the protein mols. produced by bacteria that possess antimicrobial activities and play a major role as preservative in food industry. The widely used De Man Rogosa arid Sharpe (MRS) medium for the growth of lactic acid bacteria is quite expensive and isa barrier for the large scale economical prodn. of bacteriocin. The present study alms at optimizing the medium compn. of bacteriociri prodn. using cheaper carbon and nitrogen sources. Methodol.:Lactobacillus plantarum JX1 83220, isolated from goat milkwas used for the prodn. Economical carbon and nitrogen sources such as molasses, red lentil were selected to replace the expensive dextrose and peptone resp. in the MRS Medium, The concn. of molasses as a carbon source, the concn. of red lentil as a nitrogen source and salt concn. were the three chosen process variables optimized by applying the box-behnken design of response surface methodol. so as to obtain the max. activity of bacteriocin. Results: rhe bacteriocin activityof 1839 AU wasfound at theoptimumcornbination of processvariables: Molassesconcn. 37.7 % (vol./vol.), red lentil concn. 3.97% (w/v) and NaCI concn. 1.69 g L♂1. Conclusion: Molasses and red lentil are proved to be potential cheaper sources to replace the expensive carbon and nitrogen sources in the MRS medium for the prodn. of bacteriocin from isolated Lactobacillus plantarum JX 183220. [on SciFinder(R)]