Abstract
A newly isolated mutation (Gln508Leu) and a combination of it with previously discovered beneficial mutations in polyhydroxyalkanoate synthase 1 from Pseudomonas sp. 61-3 were found to enhance the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3HB-co-3-hydroxyalkanoate)s in recombinant Escherichia coli.
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Shozui, F., Sun, J., Song, Y., Yamada, M., Sakai, K., Matsumoto, K., … Taguchi, S. (2010). A new beneficial mutation in pseudomonas sp. 61-3 Polyhydroxyalkanoate (PHA) synthase for enhanced cellular content of 3-hydroxybutyrate-based pha explored using its enzyme homolog as a mutation template. Bioscience, Biotechnology and Biochemistry, 74(8), 1710–1712. https://doi.org/10.1271/bbb.100224
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