The synthesis and characterization of porous hydroxyapatite using gelatin coating for bone scaffold application

Abstract

The writers conducted a study about the synthetic and characterization of porous hydroxyapatite with gelatin coating for bone scaffold application. The purpose was to find out the impact of gelatin concentration variation on the scaffold to get the best mechanical and physical properties to be applied as a bone scaffold according to the existing standard. Making gelatin-coated porous hydroxyapatite scaffold (HA-Gelatin) using a polymeric sponge method that is using a sponge as a mold for hydroxyapatite scaffold then sintering with a temperature reaching 1250°C for 8 hours and then coated with the gelatin concentration variations are 13 wt%, 15 wt%, 17 wt%, and 19 wt%. The characterization of mechanical and physical properties test at scaffold showed the scaffold has the characteristic which meets the standard of mechanical and physical properties for human bone. FTIR test showed an HA-Gelatin scaffold having a carbonate ion group from hydroxyapatite at wave number 873.75 cm-1, and the phosphate group at wave number 567.07 cm-1 to 962.48 cm-1, NH group from gelatin at wave number 3437.15 cm-1. According to the result, the best performance of scaffold was obtained by 19% gelatin concentration, which has a pore size value of 100-300 μm, compressive strength 6.7 MPa and porosity 67.12 %. Based on the result of the characterization, scaffold with gelatin coating has fulfilled criteria for bone regeneration.