In search of a tolerance-induction strategy for cow's milk allergies: Significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization

9Citations
Citations of this article
46Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

OBJECTIVE: To explore the use of β-lactoglobulin polymerized using microbial transglutaminase and heating to identify whether protein polymerization could reduce in vivo allergenicity and maintain in vitro and ex vivo immunoreactivity for use in tolerance-induction protocols. METHODS: Based on previous protocols applied in mice and children, we performed in vivo challenges (using a skin prick test) with native and polymerized β-lactoglobulin in adult patients with an IgE-mediated allergy to plactoglobulin. In vitro humoral immunoreactivity was analyzed using immunoblotting. Cell-mediated immunoreactivity was analyzed using ex vivo challenges with native and polymerized β-lactoglobulin and monitored by leukocyte adherence inhibition tests. RESULTS: The skin tests demonstrated that there was a significant reduction in immediate cutaneous reactivity after polymerization. Polymerization did not decrease the immunoblotting detection of s-IgE specific to β-lactoglobulin. Cell-mediated immunoreactivity, as assessed by ex vivo challenges and leukocyte adherence inhibition tests, did not exhibit significant differences between leukocytes challenged with native versus polymerized β-lactoglobulin. CONCLUSIONS: The polymerization of β-lactoglobulin decreased in vivo allergenicity and did not decrease in vitro humoral or ex vivo cell-mediated immunoreactivity. Therefore, we conclude that inducing polymerization using transglutaminase represents a promising technique to produce suitable molecules for the purpose of designing oral/ sublingual tolerance induction protocols for the treatment of allergies. © 2012 CLINICS.

Cite

CITATION STYLE

APA

Olivier, C. E., dos Santos Lima, R. P., Pinto, D. G., dos Santos, R. A. P. G., da Silva, G. K. M., Lorena, S. L. S., … de Lima Zollner, R. (2012). In search of a tolerance-induction strategy for cow’s milk allergies: Significant reduction of beta-lactoglobulin allergenicity via transglutaminase/cysteine polymerization. Clinics, 67(10), 1171–1179. https://doi.org/10.6061/clinics/2012(10)09

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free