Development and characterization of antisera specific for amino- and carboxy-terminal regions of pp60src

Abstract

Antisera to the Rous sarcoma virus transforming protein pp60src were produced in rabbits immunized with p60src purified from recombinant Escherichia coli. Immunization with isolated V8 proteolytic fragments of p60src resulted in antisera specific for the amino- and carboxy-terminal domains. The reactions of these antisera with pp60v-src and the normal cell protein p60c-src were characterized.