Vitamin E deficiency disrupts gene expression networks during Zebrafish development

14Citations
Citations of this article
21Readers
Mendeley users who have this article in their library.

Abstract

Vitamin E (VitE) is essential for vertebrate embryogenesis, but the mechanisms involved remain unknown. To study embryonic development, we fed zebrafish adults (>55 days) either VitE sufficient (E+) or deficient (E–) diets for >80 days, then the fish were spawned to generate E+ and E– embryos. To evaluate the transcriptional basis of the metabolic and phenotypic outcomes, E+ and E– embryos at 12, 18 and 24 h post-fertilization (hpf) were subjected to gene expression profiling by RNASeq. Hierarchical clustering, over-representation analyses and gene set enrichment analyses were performed with differentially expressed genes. E– embryos experienced overall disruption to gene expression associated with gene transcription, carbohydrate and energy metabolism, intracellular signaling and the formation of embryonic structures. mTOR was apparently a major controller of these changes. Thus, embryonic VitE deficiency results in genetic and transcriptional dysregulation as early as 12 hpf, leading to metabolic dysfunction and ultimately lethal outcomes.

References Powered by Scopus

Stages of embryonic development of the zebrafish

9766Citations
N/AReaders
Get full text

WebGestalt 2019: gene set analysis toolkit with revamped UIs and APIs

2062Citations
N/AReaders
Get full text

High-resolution in situ hybridization to whole-mount zebrafish embryos

2043Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Vitamin A and Vitamin E: Will the Real Antioxidant Please Stand Up?

98Citations
N/AReaders
Get full text

Aquatic Animal Nutrition: Organic Macro- and Micro-Nutrients

21Citations
N/AReaders
Get full text

Expanding role of vitamin E in protection against metabolic dysregulation: Insights gained from model systems, especially the developing nervous system of zebrafish embryos

13Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Head, B., Ramsey, S. A., Kioussi, C., Tanguay, R. L., & Traber, M. G. (2021). Vitamin E deficiency disrupts gene expression networks during Zebrafish development. Nutrients, 13(2), 1–16. https://doi.org/10.3390/nu13020468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 4

57%

Lecturer / Post doc 3

43%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 4

44%

Biochemistry, Genetics and Molecular Bi... 3

33%

Nursing and Health Professions 1

11%

Neuroscience 1

11%

Save time finding and organizing research with Mendeley

Sign up for free