Inorganic polyphosphate (polyP) is a significant regulatory and metabolic compound in yeast cells. We compared polyP content and localization, polyphosphatase activities, and transcriptional profile of polyP-related genes in industrially important methylotrophic yeasts, Hansenula polymorpha and Pichia pastoris. The increased need for phosphate, the decrease of long-chain polyP level, the accumulation of short-chain polyP, and enhanced endopolyphosphatase activity in the crude membrane fraction were observed in methanol-grown cells compared with glucose-grown cells of both species. Transcriptome analysis revealed notable differences in the expression patterns of key genes encoding proteins related to polyP metabolism. In methanol-grown cells, the genes encoding endopolyphosphatases and phosphate transporters were upregulated. The changes in polyP metabolism are probably related to the peculiarities of bioenergetics of methanol-grown cells.
CITATION STYLE
Andreeva, N., Ryazanova, L., Zvonarev, A., Trilisenko, L., Kulakovskaya, T., & Eldarov, M. (2018). Inorganic polyphosphate in methylotrophic yeasts. Applied Microbiology and Biotechnology, 102(12), 5235–5244. https://doi.org/10.1007/s00253-018-9008-3
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