Optimization of Cultural Conditions to Maximize the Efficiency of Petroleum Crude Oil-Degrading Bacteria

Abstract

Theoptimization of cultural conditions to maximize the efficiency of petroleumcrude oil bioremediation by three bacterial strains, Alcaligenes faecalis,Microbacterium oxydans and Microbacterium Paraoxydans wereisolated from Tebbin, Al Kanater charity and Agiba, respectively. The mineralsalt medium was used, pH 7.5, temperature 30 oC and 30 daysof incubation showed the highest degradation of crude oil with the isolatedthree bacterial strains. Ammonium sulphate as a sole N-source and crude oil asa sole C-sources were more suitable for crude oil degradation at theconcentration of 0.2 % and 1.0 %, respectively. Inoculum size of 0.8 % was themost suitable inoculum for the degradation. Moreover, the agitation conditionwas advantages at 100 rpm than the static condition for the crude oildegradation by the three bacterial strains.