The comet assay is an effective method for identifying DNA breaks and alkali-labile sites induced by genotoxins. Performed as a single-cell electrophoresis, this assay is especially simplistic, and the results are easily reproducible. DNA breakage can be quantitatively assessed by the induced comet tail regions, which can be measured using a variety of comet software. This protocol will finish within approximately two hours with adequate preparation, and digitized images can be taken using a confocal or standard fluorescence microscopes after staining the cell nucleus with a DNA dye.
CITATION STYLE
Walsh, K. D., & Kato, T. A. (2023). Alkaline Comet Assay to Detect DNA Damage. In Methods in Molecular Biology (Vol. 2519, pp. 65–72). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2433-3_7
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