Pseudomonas Motility and Antibiotics Resistance

Abstract

Knowledge about the biofilm formation and antibiotic resistance are resulting in identification of new targets for therapeutics against Pseudomonas infection. These one generally persist despite the use of long term antibiotic therapy. The ability of growing within a biofilm enhances their chances to protect themselves from host defenses, antibiotic therapies, and biocides. A necessary first step towards understanding the susceptibility of biofilms to antibiotics is to understand the mechanisms by which motility behavior is involved. To our knowledge, few studies had been undertaken to compare the implication of swimming-swarming and biofilm in antibiotics resistance. The relationship between these formations is debated in some Pseudomonas species literature. It should be noted that P. aeruginosa has been wellstudied as a model organism for the study of these interactions. By contrast, biofilms formation and motility behavior in P. fluorescens has not been extensively analysed. Our data demonstrate that our isolates exhibited an important biofilm mass and were categorized as slime-producers. The morphological and microscopic analysis of biofilm formation in these isolates revealed a very complex, dynamic and biologically exciting view about the architecture. The results indicate that biofilm formation, swarming and swimming motility exhibited a significant effect of resistance toward the β-lactam antibiotics and there is an induced swarming tendril tip bacteria phenotype with the presence of some β-lactam antibiotics.