Effects of exposure to ammonium and transplant shock upon the induction of nitrate absorption

Abstract

In barley (Hordeum vulgare L. cv Steptoe) seedlings, the time course for induction of root nitrate absorption varied significantly with pretreatment. Net nitrate uptake of nitrogen-deprived plants more than doubled during the 12 hours after first exposure to nitrate. For these plants, gentle physical disturbance of the roots inhibited net nitrate absorption for more than 6 hours and potassium absorption for 2 hours. Pretreatment with ammonium appeared sufficient to induce nitrate absorption; plants either grown for 2 weeks on or exposed for only 10 hours to a medium containing ammonium as a sole nitrogen source showed high rates of net nitrate uptake when first shifted to a medium containing nitrate. Gentle physical manipulation of these plants inhibited nitrate absorption for 2 hours and potassium absorption for more than 12 hours. These results indicate (a) that experimental protocols should avoid physical manipulation of the roots whenever possible and (b) that ammonium or a product of ammonium assimilation can induce nitrate absorption.