Different pairs of adjacent sub-regions of rat hippocampal neurons were co-cultured within two-well devices on micro-electrode arrays (MEAs). Micro-tunnels allowed passage of axons, strong structural connectivity, and information transmission between wells. Paired-pulse stimuli were delivered at each electrode to increase the probability of the excitation without overstimulation. Because of the micro-channel construction, the electrodes inside these tunnels pick up more activity than those within each well. In particular, the strong axonal inputs from the sources of native feed-forward EC-DG and CA3-CA1 co-cultures evoked more target activity than the feed-back networks. In contrast, the evoked target activity in the native feed-forward DG-CA3 networks was lower than the reverse flow of information.
CITATION STYLE
Poli, D., DeMarse, T. B., Brewer, G. J., & Wheeler, B. C. (2017). Inter-regional dynamics in hippocampal sub-networks co-cultured on micro-electrode arrays and connected via micro-tunnels. In IFMBE Proceedings (Vol. 65, pp. 651–654). Springer Verlag. https://doi.org/10.1007/978-981-10-5122-7_163
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