Abstract
The determination of the lipid content in liver offers the potential to investigate metabolic alterations in different research contexts. Here, we describe a method to determine cholesterol, triglycerides, and phospholipids in liver samples based on total lipid isolation by a 2:1 chloroform–methanol mixture (Folch extraction) and specific enzymatic colorimetric microassays in plate.
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Minniti, M. E., Ahmed, O., & Pedrelli, M. (2020). Enzymatic Quantification of Liver Lipids After Folch Extraction. In Methods in Molecular Biology (Vol. 2164, pp. 101–108). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0704-6_11
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