Development and validation of RP-HPLC-PDA method for the quantification of eugenol in developed nanoemulsion gel and nanoparticles

Abstract

Background: Eugenol is a potent phytochemical, and a plethora of delivery systems for this bioactive agent is being developed. Reversed-phase high-pressure liquid chromatography equipped with photodiode array detector (RP-HPLC-PDA) method is very useful in the quantification of the phytochemicals. Methods: The RP-HPLC-PDA system with C18 reversed-phase column (250???4.6?mm, particle size 5??m) was used in this study. Acetonitrile and water in 1:1 (v/v) ratio was chosen as the mobile phase under a column temperature of 25?C. The detection wavelength was set at 280?nm with a flow rate of 1?mL/min. Method validation was performed according to the International Conference on Harmonization guidelines. Results: HPLC method for the quantification of eugenol was successfully developed and validated. The method was validated in terms of linearity and range, accuracy, precision, specificity, robustness, detection limit, and quantitation limit. Conclusions: The developed RP-HPLC-PDA could be successfully employed for the quantification of eugenol in nanoemulsion gel and nanoparticles.