For structural, biochemical or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the “tRNA scaffold” method to RNA/protein co-expression in order to express and purify RNA by affinity in native condition. As a proof-of-concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.
CITATION STYLE
El Khouri, M., Catala, M., Seijo, B., Chabal, J., Tisné, C., Dardel, F., & Ponchon, L. (2015). Expression and purification of RNA–protein complexes in Escherichia coli. Methods in Molecular Biology, 1316, 25–31. https://doi.org/10.1007/978-1-4939-2730-2_3
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