The isolation of (6S, 9S)-cyclo(prolylvalyl) from marine actinomycete, by use of high speed countercurrent chromatography

Abstract

The marine actinomycete B 1758 came from the actinomycete collection of the Alfred Wegener Institute for Polar and Marine Research in Bremerhafen, Germany. 1.079 g of raw extract was obtained by fermentation. Seven fractions were separated by column chromatography on silica gel. Fraction 4 was separated by high speed countercurrent chromatography. Fraction 4.6 yielded 5.2 mg of (65, 95)-cyclo(prolylvalyl).