Laser microdissection (LMD) technology has been widely applied to plant tissues, offering novel information on the role of different cell-type populations during plant–microbe interactions. In this chapter, protocols to apply the LMD approach to study plant and fungal transcript profiles in different cell-type populations from arbuscular mycorrhizal (AM) roots are described in detail, starting from the biological material preparation to gene expression analyses by RT-PCR and RT-qPCR.
CITATION STYLE
Balestrini, R., & Fiorilli, V. (2020). Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis. In Methods in Molecular Biology (Vol. 2146, pp. 171–184). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0603-2_13
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