Flow cytometry: Principles and applications

Abstract

History, development and basic principles of flow cytometry Cell preparation techniques for live and lysed whole blood and antibodies Methods for determining absolute cell counts Flow cytometry forms an integral part of both basic biological research and clinical diagnosis in pathology. This straightforward new volume provides a clear, easy-to-read, and practical manual for both clinicians and non-clinicians at all levels of their careers.The chapter topics range from basic principles to more advanced subjects, such as apoptosis and cell sorting. Throughout the book, well-informed expert contributors present theoretical descriptions and practical protocols on this important and complex laboratory technique and its applications. Immunologists and Hematologists in the field of pathology, as well as biological researchers working with both human and animal models will appreciate the simple, clear-cut style in which principles and protocols in this volume are presented, and will refer to this book time and time again for clear and easy-to-follow protocols.