Synthesis and immunological evaluation of virus-like particle-milbemycin A3/A4 conjugates

Abstract

Milbemycins are macrolide antibiotics with a broad spectrum of nematocidal, insecticidal, and acaricidal activity. To obtain milbemycin A3/A4 derivatives suitable for chemical conjugation to protein carriers (milbemycin haptens), succinate linker and a novel 17-atom-long linker containing a terminal carboxylic acid group were attached to the milbemycin core in a protecting group-free synthesis. The obtainedmilbemycin A3/A4 derivativeswere coupled to Potato virus Y-like nanoparticles by the activated estermethod. The reaction products were characterized and used inmice immunization experiments. It was found that the mice developed weak specific immune responses toward all tested milbemycin haptens.