The interplay between excitation and inhibition is crucial for neuronal circuitry in the brain. Inhibitory cell fractions in the neocortex and hippocampus are typically maintained at 15 to 30%, which is assumed to be important for stable dynamics. We have studied systematically the role of precisely controlled excitatory/inhibitory (E/I) cellular ratios on network activity using mice hippocampal cultures. Surprisingly, networks with varying E/I ratios maintain stable bursting dynamics. Interburst intervals remain constant for most ratios, except in the extremes of 0 to 10% and 90 to 100% inhibitory cells. Single-cell recordings and modeling suggest that networks adapt to chronic alterations of E/I compositions by balancing E/I connectivity. Gradual blockade of inhibition substantiates the agreement between the model and experiment and defines its limits. Combining measurements of population and single-cell activity with theoretical modeling, we provide a clearer picture of how E/I balance is preserved and where it fails in living neuronal networks.
CITATION STYLE
Sukenik, N., Vinogradov, O., Weinreb, E., Segal, M., Levina, A., & Moses, E. (2021). Neuronal circuits overcome imbalance in excitation and inhibition by adjusting connection numbers. Proceedings of the National Academy of Sciences of the United States of America, 118(12). https://doi.org/10.1073/pnas.2018459118
Mendeley helps you to discover research relevant for your work.