The assay of abscisic acid-induced stomatal movement in leaf senescence

Abstract

Abscisic acid (ABA) is a sesquiterpenoid (15-carbon) hormone that comprehensively regulates plant stress responses, development, and senescence. Stomata are epidermal pores on plant surface used for exchanging gases such as carbon dioxide, water vapor, and oxygen. One of the mechanisms that ABA regulates leaf senescence is to control stomatal movement and thus water loss during leaf senescence. Here we describe the procedure of measuring stomatal movement in response to ABA treatments, which will provide a useful protocol to investigate ABA signaling in leaf senescence.