Used pollen from corn, ground with a buffer to a viscous material and extracted four times in cold acetone to remove fats, centrifuged, dried, fractioned by ammonium sulfate and run chromatographically to isolate actin.
CITATION STYLE
Yen, L.-F., Liu, X., & Liu, G. (1992). In Vitro Polymerization of F-actin and Assembly of Microfilament Cytoskeleton of Pollen Actin. In Angiosperm Pollen and Ovules (pp. 43–52). Springer New York. https://doi.org/10.1007/978-1-4612-2958-2_9
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