Large-scale femtoliter droplet array as a platform for single cell efflux assay of bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single bacterial cells, fluorescence-based detection of efflux activity at the single cell level, and collection of single cells from droplet and subsequent gene analysis are described in detail.
CITATION STYLE
Iino, R., Sakakihara, S., Matsumoto, Y., & Nishino, K. (2018). Large-scale femtoliter droplet array for single cell efflux assay of bacteria. In Methods in Molecular Biology (Vol. 1700, pp. 331–341). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7454-2_18
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