The mitochondrial membrane potential is the dominant component of the proton-motive force that is the potential term in the proton circuit linking electron transport to ATP synthesis and other energy-dependent mitochondrial processes. Cationic fluorescent probes have been used for many years to detect gross qualitative changes in mitochondrial membrane potentials in intact cell culture. In this chapter, I describe how these fluorescence signals may be used to obtain a semiquantitative measure of changes in mitochondrial membrane potential. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Nicholls, D. G. (2012). Fluorescence measurement of mitochondrial membrane potential changes in cultured cells. Methods in Molecular Biology, 810, 119–133. https://doi.org/10.1007/978-1-61779-382-0_8
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