Alcohol dehydrogenase (Adh) enzyme is a potent biochemical marker for submergence tolerance in rice (Oryza sativa L.) during seedling stage of growth

Abstract

Aim: The incorporation of sub 1 gene in high yielding rice mega genotypes indicate that higher amount of Adh enzyme synthesis is controlled by the genetic factors. Adh enzyme will be the one of the vital factor for underwater respiration process of survival. Hence, rice genotypes with high amount of alcohol dehydrogenase enzyme synthesis during anoxic condition in compare with tolerant checks could be good promise for early introduction to the submergence prone area. Methodology: Fresh and disease free seeds of fifty diverse rice genotypes including tolerant and susceptible checks were taken and germinated. 0.1 gm cut five days old rice seedlings of various submergence dose of day 1, 2, 3, 4 and 5 along with control were evaluated separately in three replications for Adh enzyme estimation by standard protocols with spectrophotometer. Results: The peak time of Adh enzyme synthesis was during 72 hours to 96 hour of submergence period. Among paddy genotypes Mahananda along with Sabita and Purnendu performed well in compare with tolerant check paddy genotypes. Conclusion: As it is controlled by genetic factors, so estimation of Adh enzyme activity under submerged stage could be the potent biochemical marker for the screening for paddy genotypes for seedling stage of crop growth during flash flooding condition.