Mitochondrial tRNA 5′-editing in Dictyostelium discoideum and Polysphondylium pallidum

Abstract

Background: Secondary structure modeling predicts 5′-editing of mitochondrial tRNAs in amoebozoans Dictyostelium discoideum and Polysphondylium pallidum. Results: Experimental verification reveals unexpected instances of editing and species-specific variations. Conclusion: Mitochondrial tRNA 5′-editing is a complex process in which 3′-5′ RNA polymerases play a key role. Significance: Further biochemical characterization of mitochondrial tRNA 5′-editing reactions and enzymes is indicated. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.